Western blot analysis of HMCES was performed by loading whole cell lysate in 1X LDS sample buffer with 2-ME from 5 x 105 mouse transformed pre-B cells transduced with or without guide RNA targeting HMCES (gHMCES). Samples were loaded onto a 4-12 % Bis-Tris polyacrylamide gel (Product # NP0336BOX). Proteins were transferred to nitrocellulose membrane with wet/tank transfer. Membrane was blocked in 5% milk/TBST. The membrane was probed with a polyclonal anti-HMCES antibody (Product # PA5-70309) at a dilution of 1:1,000 in 2% milk/TBST at 4 deg overnight, followed by a secondary antibody HRP-anti-rabbit at a dilution of 1:5,000 at room temperature for 1 hour. Chemiluminescent detection was performed. Data courtesy of Antibody Exchange Program.
Supplier Page from Thermo Fisher Scientific for HMCES Antibody